July 16th, 2012
•Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.
Related Videos
Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
Assays for the Identification of Novel Antivirals against Bluetongue Virus
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved