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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

26.5K Views

12:03 min

October 31st, 2012

DOI :

10.3791/4327-v

October 31st, 2012


Transcript

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Human Induced Pluripotent Stem Cells

Chapters in this video

0:05

Title

1:50

Isolation and Expansion of PBMCs

4:34

Transduction of PBMCs with STEMCCA Lentivirus

6:11

Plating Transduced Cells onto MEFs

8:41

Picking and Expansion of iPSC Clones

9:26

Results: Human Induced Pluripotent Stem Cells

11:26

Conclusion

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