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Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

DOI :

10.3791/4441-v

October 5th, 2012

October 5th, 2012

11,802 Views

1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

Described is a two-step labeling process using β-glucosyltransferase (β-GT) to transfer an azide-glucose to 5-hmC, followed by click chemistry to transfer a biotin linker for easy and density-independent enrichment. This efficient and specific labeling method enables enrichment of 5-hmC with extremely low background and high-throughput epigenomic mapping via next-generation sequencing.

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Selective Capture

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