October 5th, 2012
•Described is a two-step labeling process using β-glucosyltransferase (β-GT) to transfer an azide-glucose to 5-hmC, followed by click chemistry to transfer a biotin linker for easy and density-independent enrichment. This efficient and specific labeling method enables enrichment of 5-hmC with extremely low background and high-throughput epigenomic mapping via next-generation sequencing.
Tags
Related Videos
Isolation of Genomic DNA from Mouse Tails
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA
Competitive Genomic Screens of Barcoded Yeast Libraries
Extraction of High Molecular Weight DNA from Microbial Mats
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved