Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
2.1K Views
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07:15 min
•
November 22nd, 2021
DOI :
November 22nd, 2021
•Xiaoyan Chang*1, Keru Zhou*1, Yanguang Liu1,2, Lian Duan3, Lichun Zhang2, Guoliang Zhang3, Haikun Wang4, Guiqin Wang1
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Chapters in this video
0:04
Introduction
0:40
Pseudovirus Packaging with Calcium‐Phosphate Transfection
1:33
Detection of the HA, NA, and HIV‐1 P24 Protein Expression of Influenza Pseudovirus
2:24
Pseudovirus Titration
3:22
Luciferase Assay
4:06
Pseudovirus Neutralization (PN) Assay
5:16
Results: HPAI H5 Pseudovirus Preparations and Pseudovirus Neutralization Assays
6:50
Conclusion
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