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Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

DOI :

10.3791/51953-v

September 25th, 2014

September 25th, 2014

11,580 Views

1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.

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Keywords Homalodisca Vitripennis

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