November 11th, 2014
•Yeast proteinopathy models are valuable tools to assess the toxicity and aggregation of proteins implicated in disease. Here, we present methods for screening Hsp104 variant libraries for toxicity suppressors. This protocol could be adapted to screen any protein library for toxicity suppressors of any protein that is toxic in yeast.
Tags
Related Videos
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System
Isolating Stem Cells from Soft Musculoskeletal Tissues
Yeast Colony Embedding Method
Principles of Rodent Surgery for the New Surgeon
Manual Restraint and Common Compound Administration Routes in Mice and Rats
High-throughput Yeast Plasmid Overexpression Screen
Purification of Hsp104, a Protein Disaggregase
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved