Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

25.4K Views

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13:47 min

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February 24th, 2015

DOI :

10.3791/52246-v

February 24th, 2015

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Francine E. Garrett-Bakelman*¹, Caroline K. Sheridan*¹, Thadeous J. Kacmarczyk¹, Jennifer Ishii¹, Doron Betel¹^,², Alicia Alonso¹, Christopher E. Mason³, Maria E. Figueroa⁴, Ari M. Melnick¹

¹Department of Medicine, Weill Cornell Medical College, ²Institute for Computational Biomedicine, Weill Cornell Medical College, ³Department of Physiology and Biophysics, Weill Cornell Medical College, ⁴Department of Pathology, University of Michigan

Summary

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DNA Methylation

Bisulfite Sequencing

Reduced Representation

ERRBS

Base Pair Resolution

Quantitative Methylation

Genomic Loci

Restriction Enzyme

Next generation Sequencing

Bioinformatics Analysis

Chapters in this video

0:05

Title

2:22

Adapter Ligation and DNA Size-selection via Gel Purification

7:32

Bisulfite Conversion, Enrichment PCR, and DNA Purification

9:40

Library Preparation for Sequencing

10:42

Results: ERRBS Yields Base Pair Resolution CpG Methylation Data

12:15

Conclusion

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