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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

10.2K Views

10:18 min

April 25th, 2015

April 25th, 2015

10,217 Views

0:05

Title

1:21

Isolation and Cleanup of Total RNA

2:58

cDNA Preparation and Labeling

4:54

cDNA Hybridization

6:59

Slide Washing

8:02

Results: Bacterial Transcriptome Analysis through Serine-dependent Gene Regulation

9:39

Conclusion

This manuscript describes the use of state-of-the-art technology provided by DNA-microarrays. Microarrays provide an overview of the transcriptomic changes in bacteria incurred under a specific condition. Moreover, we highlight the ease by which large amounts of data can be analyzed by using convenient in-house developed software packages.

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