JoVE Logo
Faculty Resource Center

Sign In

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

DOI :

10.3791/53429-v

7:48 min

November 21st, 2015

November 21st, 2015

17,192 Views

1European Cancer Stem Cell Research Institute, Cardiff University, 2Institut Curie

Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.

Tags

Paneth Cells

-- Views

Related Videos

article

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

article

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

article

Live Imaging of Mouse Secondary Palate Fusion

article

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

article

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

article

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

article

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

article

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

article

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

article

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved