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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

DOI :

10.3791/53586-v

October 11th, 2015

October 11th, 2015

11,462 Views

1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

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Keywords Meiotic Spindle

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