Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
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09:23 min
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March 26th, 2016
DOI :
March 26th, 2016
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Chapters in this video
0:05
Title
0:42
Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining
1:44
RNA Probe Synthesis
4:12
Cryosectioning and Hybridization
5:33
Visualization of the FITC Probe and the DIG Probe
7:23
Immunohistochemistry and Mounting
8:16
Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum
8:45
Conclusion
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