Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining
1:44
RNA Probe Synthesis
4:12
Cryosectioning and Hybridization
5:33
Visualization of the FITC Probe and the DIG Probe
7:23
Immunohistochemistry and Mounting
8:16
Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum
8:45
Conclusion
필기록
The overall goal of this experiment is to simultaneously detect the expression of three different genes in brain sections, using double fluorescence in situ hybridization followed by immunostaining. This method can help answer key questions in the
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.