JoVE Logo
Faculty Resource Center

Sign In

Studying Protein Import into Chloroplasts Using Protoplasts

DOI :

10.3791/58441-v

December 10th, 2018

December 10th, 2018

9,147 Views

1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology

Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.

-- Views

Related Videos

article

mRNA Interactome Capture from Plant Protoplasts

article

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

article

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved