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Studying Protein Import into Chloroplasts Using Protoplasts

DOI :

10.3791/58441-v

December 10th, 2018

December 10th, 2018

9,290 Views

1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology

Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.

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Protein Import

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