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Quantitative PCR of T7 Bacteriophage from Biopanning

DOI :

10.3791/58165-v

September 27th, 2018

September 27th, 2018

10,105 Views

1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

A reproducible, accurate, and time efficient quantitative PCR (qPCR) method to enumerate T7 bacteriophage is described here. The protocol clearly describes phage genomic DNA preparation, PCR reaction preparation, qPCR cycling conditions, and qPCR data analysis.

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