Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
1:05
Site-directed Mutagenesis of pGEX-Nrf2
3:45
In Vitro Competitive 5' Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
6:13
In Vitro AMPK Activity Assay with a Site-specific Mutant
Transcript
The overall goal of this procedure is to simultaneously assess multiple potential phosphorylation sites and to identify sites for subsequent validation by phosphorylation site mutants. This method can help answer key questions in the biochemistry
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Peptidkonkurranseanalyser er mye brukt i en rekke molekylære og immunologiske eksperimenter. Dette papiret beskriver en detaljert metode for en in vitro oligopeptid-konkurrerende kinaseanalyse og de tilhørende valideringsprosedyrer, som kan være nyttige for å finne spesifikke fosforyleringssteder.