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Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

DOI :

10.3791/55747-v

July 23rd, 2017

July 23rd, 2017

9,226 Views

1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.

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Purification

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