Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

8.0K Views

10:56 min

August 1st, 2019

DOI :

10.3791/59763-v

August 1st, 2019


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Dual Bioluminescence Imaging

Chapters in this video

0:04

Title

1:33

Lentivirus-Renilla-Luciferin-Red Fluorescent Protein (LV-Rluc-RFP; RR) and LV-Rluc-RFP-Herpes Simplex Virus Truncated Thymidine Kinase (HSV-ttk) (LV-Rluc-RFP-TTK; RT) Packaging and Production

3:38

LV-RR and LV-RRT Lentiviral Transduction for Gene Expression in 4T1 Cells

4:37

Drug Screening and LV-RR and LV-RRT-Transduced 4T1 Cell Identification

5:18

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Firefly Luciferase (Fluc)-Knockin (KI) Mice and Tumor-Bearing Mouse Model

6:50

Dual Bioluminescence Tumor (Rluc) and Angiogenesis (Fluc) Imaging

7:58

Results: Representative In Situ Tumor Growth and Angiogenesis Visualization and Analysis

9:52

Conclusion

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