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Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

12.3K Views

10:37 min

May 10th, 2018

DOI :

10.3791/57519-v

May 10th, 2018


Transcript

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Toeprinting Analysis

Chapters in this video

0:04

Title

0:34

Preparation of mRNA

2:59

Toeprinting Assay

6:32

Sequencing Reactions and Gel Electrophoresis

7:53

Results: Importance of IRES Secondary Structure in the Formation of Translation Initiation Complexes on an Uncapped XIAP IRES RNA

9:35

Conclusion

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