Mating Between Cells Containing Library Genes and Query Yeast
8:20
Spotting Assay
9:12
Results: Successful Screening for Genes that Modify FUS Toxicity
10:44
Conclusion
Transcript
This method can help answer key questions in the neurodegenerative disease field, such as what genetic factors and pathways regulate the toxicity of disease-associated proteins. The main advantage of this technique is that the highly efficient pro
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This article presents a mating-based method to facilitate overexpression screening in budding yeast using an arrayed plasmid library.