JoVE Logo
Faculty Resource Center

Sign In

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

DOI :

10.3791/58366-v

September 27th, 2018

September 27th, 2018

7,574 Views

1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

Tags

Oral Intubation

-- Views

Related Videos

article

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

article

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

article

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

article

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

article

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

article

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

article

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

article

A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

article

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

article

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved