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Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

DOI :

10.3791/58366-v

September 27th, 2018

September 27th, 2018

7,490 Views

1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

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