JoVE Logo
Faculty Resource Center

Sign In

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

DOI :

10.3791/58387-v

November 30th, 2018

November 30th, 2018

10,798 Views

1Veterinary Medicine Teaching and Research Center, University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences, University of California Davis

Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.

Tags

Liver Mitochondrial Oxygen Consumption

-- Views

Related Videos

article

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

article

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

article

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

article

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

article

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

An Improved Method to Isolate Mitochondrial Contact Sites

article

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry

article

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved