Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

9.1K Views

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08:01 min

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November 20th, 2018

DOI :

10.3791/58467-v

November 20th, 2018

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Dan Zhou*¹^,², Lei Tan*¹, Jian Li*³, Tanbin Liu¹, Yi Hu¹, Yalan Li¹, Sachiyo Kawamoto⁴, Chengyu Liu⁵, Shiyin Guo³, Aibing Wang¹

¹Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), ²Department of Pathology, Georgetown University Medical School, ³College of Food Science and Technology, Hunan Agricultural University (HUNAU), ⁴Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), ⁵Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)

Summary

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Keywords Homologous Recombination

Mouse Embryonic Stem Cells

Southern Blotting

Polymerase Chain Reaction

Molecular Biology

Cellular Biology

Genetically Modified Cells

GDNA Digestion

Agarose Gel Electrophoresis

DNA Transfer

DNA Labeling

DNA Hybridization

Chapters in this video

0:04

Title

1:02

Southern Blotting Screening

4:40

Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells

6:07

Conclusion

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