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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

DOI :

10.3791/59387-v

September 5th, 2019

September 5th, 2019

7,932 Views

1Department of Chemistry, University of Virginia, 2Department of Molecular Physiology & Biological Physics, University of Virginia School of Medicine

3D single-molecule localization microscopy is utilized to probe the spatial positions and motion trajectories of fluorescently labeled proteins in living bacterial cells. The experimental and data analysis protocol described herein determines the prevalent diffusive behaviors of cytosolic proteins based on pooled single-molecule trajectories.

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Single Molecule Tracking Microscopy

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