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Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

9.5K Views

09:57 min

August 12th, 2019

DOI :

10.3791/59706-v

August 12th, 2019


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Monocyte Chemotactic Activity

Chapters in this video

0:04

Title

0:47

Preparation of the Monocyte Chemoattractant Protein (MCP)-1-loaded Growth Factor-reduced Basement Membrane-derived Solution

1:40

Basement Membrane-derived Solution Injection and Gel Plug Harvest

3:29

Basement Membrane-derived Gel Plug Digestion

4:43

Single-cell Western Blot (scWB) Analysis

6:38

scWB Chip Stripping

7:35

Results: Representative Basement Membrane-derived Gel Plug Cell Recruitment Analyses

9:22

Conclusion

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