Intravital microscopy of the small intestinal mucosa can provide insight into the spatiotemporal dynamics of cellular interactions within the epithelium between different immune cell types, or between these two compartments. The main advantage of
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We describe a method to visualize GFP-labeled γδ IELs using intravital imaging of murine small intestine by inverted spinning disk confocal microscopy. This technique enables the tracking of live cells within the mucosa for up to 4 h and can be used to investigate a variety of intestinal immune-epithelial interactions.