Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
7.1K Views
•
08:30 min
•
January 31st, 2020
DOI :
January 31st, 2020
•1Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, 2Department of Urology, Medical University of South Carolina, 3Department of Urology, College of Medicine, University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center
Explore More Videos
Chapters in this video
0:04
Introduction
1:45
Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
3:28
Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
5:52
Results: Investigation of Ion Channel Properties in Isolated DSM Cells
7:07
Conclusion
Related Videos
10:45
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10.0K Views
17:41
Focal Ca2+ Transient Detection in Smooth Muscle
11.2K Views
14:39
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
17.2K Views
10:26
Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology
26.2K Views
06:01
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
5.6K Views
10:53
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
20.3K Views
06:36
A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
7.1K Views
05:35
Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
991 Views
06:22
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
3.0K Views
02:06
Amphotericin-B Mediated Perforated Patch-Clamp Analysis: An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells
1.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved