Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

7.4K Views

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09:11 min

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September 25th, 2019

DOI :

10.3791/60195-v

September 25th, 2019

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7,447 Views

Aleksandra Klimas¹, Octavian Bucur², Brigdet Njeri¹, Yongxin Zhao¹

¹Department of Biological Sciences, Carnegie Mellon University, ²QPathology

Chapters

0:05

Title

1:19

Preparation of Archived and Freshly Prepared Clinical Tissue Slides for ExPath

4:05

In Situ Polymerization of Specimens

5:16

Sample Digestion and Imaging

6:40

Results: Multicolor Super-resolution Tissue Imaging using Expansion Pathology

8:18

Conclusion

Transcript

We are presenting expansion pathology, or ExPath for short, a low cost measure to image biomolecule of interest in the standard clinical tissue sections, with nanoscale precision. ExPath circumvents the diffraction limit of conventional light micr

Summary

Nanoscale imaging of clinical tissue samples can improve understanding of disease pathogenesis. Expansion pathology (ExPath) is a version of expansion microscopy (ExM), modified for compatibility with standard clinical tissue samples, to explore the nanoscale configuration of biomolecules using conventional diffraction limited microscopes.

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