Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

7.4K Views

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09:11 min

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September 25th, 2019

DOI :

10.3791/60195-v

September 25th, 2019

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7,442 Views

Aleksandra Klimas¹, Octavian Bucur², Brigdet Njeri¹, Yongxin Zhao¹

¹Department of Biological Sciences, Carnegie Mellon University, ²QPathology

Chapitres

0:05

Title

1:19

Preparation of Archived and Freshly Prepared Clinical Tissue Slides for ExPath

4:05

In Situ Polymerization of Specimens

5:16

Sample Digestion and Imaging

6:40

Results: Multicolor Super-resolution Tissue Imaging using Expansion Pathology

8:18

Conclusion

Transcription

We are presenting expansion pathology, or ExPath for short, a low cost measure to image biomolecule of interest in the standard clinical tissue sections, with nanoscale precision. ExPath circumvents the diffraction limit of conventional light micr

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Résumé

Nanoscale imaging of clinical tissue samples can improve understanding of disease pathogenesis. Expansion pathology (ExPath) is a version of expansion microscopy (ExM), modified for compatibility with standard clinical tissue samples, to explore the nanoscale configuration of biomolecules using conventional diffraction limited microscopes.

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