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JoVE Journal

Biochemistry

This content is Open Access.

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

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08:26 min

December 10th, 2019

December 10th, 2019

8,862 Views

0:04

Title

0:59

Silicon Nitride (Si3N4) Membrane Support Preparation

2:14

Cell Seeding

3:04

Plunge-Freezing

4:46

Plunge-Frozen Cell Freeze-Drying

6:05

Results: Representative X-Ray Fluorescence Imaging of Frozen Hydrated and Freeze-Dried Cells

7:28

Conclusion

Transcript

Presented here is a protocol for cell culture on silicon nitride membranes and plunge-freezing prior to X-ray fluorescence imaging with a synchrotron cryogenic X-ray nanoprobe. When only room temperature nano-analysis is provided, the frozen samples can be further freeze-dried. These are critical steps to obtain information on the intracellular elemental composition.

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