Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

4.0K Views

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11:55 min

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May 28th, 2021

DOI :

10.3791/62274-v

May 28th, 2021

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Nina Vyas*¹, Nina Perry*¹, Chidinma A. Okolo¹, Ilias Kounatidis¹, Thomas M. Fish¹, Kamal L. Nahas¹^,², Archana Jadhav¹, Mohamed A. Koronfel¹, Johannes Groen³, Eva Pereiro³, Ian M. Dobbie⁴, Maria Harkiolaki¹

¹Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, ²Division of Virology, Department of Pathology, University of Cambridge, ³ALBA Synchrotron, Beamline 09 - MISTRAL, ⁴Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford

Summary

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Cryo Structured Illumination Microscopy

Super Resolution Imaging

Cellular Ultrastructure Dynamics

Vaccine Production

Quality Control

Post market Surveillance

Antibody Engineering

Nano Particle Characterization

Data Collection

Liquid Nitrogen Handling

Chapters in this video

0:04

Introduction

1:09

Preparation of the Cryo-Stage

2:08

Transfer of the Sample Storage Box into the Cryo-Stage

3:34

Stage Docking and Focusing

5:03

Brightfield Mosaic Acquisition and Identification of Areas of Interest

6:48

Data Collection

8:59

After Imaging

9:38

Results: Cryo-structured Illumination Microscopic Imaging

11:00

Conclusion

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