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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

DOI :

10.3791/62274-v

11:55 min

May 28th, 2021

May 28th, 2021

3,671 Views

1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford

This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.

Tags

Cryo Structured Illumination Microscopy

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