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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

DOI :

10.3791/62107-v

7:27 min

April 9th, 2021

April 9th, 2021

3,876 Views

1Department of Biology, University of Waterloo

The study of fatty acylation has important implications in cellular protein interactions and diseases. Presented here is a modified protocol to improve click chemistry detection of fatty acylated proteins, which can be applied in various cell types and combined with other assays, including pulse-chase and mass spectrometry.

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Alkynyl Fatty Acid Analogs

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