Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
5.1K Views
•
09:22 min
•
May 12th, 2020
DOI :
May 12th, 2020
•Katarzyna Okurowska1,2, Sanhita Roy3, Praveen Thokala4, Lynda Partridge1,5, Prashant Garg3, Sheila MacNeil1,6, Peter N. Monk1,7, Esther Karunakaran1,2
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, 2Department of Chemical and Biological Engineering, University of Sheffield, 3Hyderabad Eye Research Foundation, L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, 5Department of Molecular Biology and Biotechnology, University of Sheffield, 6Department of Materials Science and Engineering, University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield
Chapters in this video
0:05
Introduction
0:35
Preparation of the Corneoscleral Button
2:45
Maintenance of the Corneoscleral Buttons
3:29
Preparation of an Inoculum
4:17
Infecting the Corneoscleral Button
5:54
Homogenization of the Cornea
7:21
Results: Ex Vivo Model Mimics In Vivo Infection
8:35
Conclusion
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