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A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

DOI :

10.3791/60171-v

October 6th, 2019

October 6th, 2019

6,868 Views

1Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), 2University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen

Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.

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Keywords Porcine Corneal Endothelial Organ Culture

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