Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
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06:27 min
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September 12th, 2020
DOI :
September 12th, 2020
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Chapters in this video
0:00
Introduction
0:42
Maintenance, Synchronization and Preparation of Transgenic Nematodes for Monitoring de novo Protein Synthesis
2:11
FRAP Assay using Transgenic Animals Expressing Somatic Tissue Reporters plfe-2GFP and psod-3GFP
3:01
Mounting the Samples and Perform FRAP Assay using Transgenic Nematodes Expressing Pan-Neuronally Cytoplasmic GFP, punc-119GFP
4:19
Representative Results: In Vivo Assessment of de novo Protein Synthesis in C. elegans
5:46
Conclusion
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