Maintenance, Synchronization and Preparation of Transgenic Nematodes for Monitoring de novo Protein Synthesis
2:11
FRAP Assay using Transgenic Animals Expressing Somatic Tissue Reporters plfe-2GFP and psod-3GFP
3:01
Mounting the Samples and Perform FRAP Assay using Transgenic Nematodes Expressing Pan-Neuronally Cytoplasmic GFP, punc-119GFP
4:19
Representative Results: In Vivo Assessment of de novo Protein Synthesis in C. elegans
5:46
Conclusion
필기록
The rate of protein synthesis is perturbed during disease and aging. Fluorescence recovery after photobleaching, or FRAP, allows the measurement of rate of protein synthesis in vivo. We use transparent C.elegans worms, which express GFP, as a mode
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Here, we introduce and describe a nonradioactive and noninvasive method to assess de novo protein synthesis in vivo, utilizing the nematode Caenorhabditis elegans and fluorescence recovery after photobleaching (FRAP). This method can be combined with genetic and/or pharmacological screens to identify novel modulators of protein synthesis.