JoVE Logo
Faculty Resource Center

Sign In

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

DOI :

10.3791/63258-v

6:48 min

April 29th, 2022

April 29th, 2022

1,810 Views

1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

We present a method for evaluating microRNA expression in the kidney and serum of mice with age-dependent renal impairment by quantitative reverse-transcription polymerase chain reaction.

Tags

Quantitative Real Time Polymerase Chain Reaction

-- Views

Related Videos

article

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

article

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

article

Exercise Test for Evaluation of the Functional Efficacy of the Pig Cardiovascular System

article

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

article

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

article

Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains

article

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates

article

Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved