Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
3.9K Views
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07:34 min
•
September 22nd, 2020
DOI :
September 22nd, 2020
•Yangyang Deng*1,2, Ming Zhou*1,3, Ying Lu1, Jiao Chen1, Zuhui Pu4, Dongjing Yu1,5, Yifan Dai6, Yongqiang Zhan7, Lisha Mou1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
Chapters in this video
0:04
Introduction
0:52
Isolating Genomic DNA
2:20
Verification of Primer Specificity
3:42
Standard Curve of cpsDNA
4:45
Quantitation of Circulating Pig-specific DNA
5:55
Results: PCR Validation of Porcine Specific Primers
6:51
Conclusion
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