Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
3.9K Views
•
07:34 min
•
September 22nd, 2020
DOI :
September 22nd, 2020
•Yangyang Deng*1,2, Ming Zhou*1,3, Ying Lu1, Jiao Chen1, Zuhui Pu4, Dongjing Yu1,5, Yifan Dai6, Yongqiang Zhan7, Lisha Mou1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
Chapters in this video
0:04
Introduction
0:52
Isolating Genomic DNA
2:20
Verification of Primer Specificity
3:42
Standard Curve of cpsDNA
4:45
Quantitation of Circulating Pig-specific DNA
5:55
Results: PCR Validation of Porcine Specific Primers
6:51
Conclusion
Related Videos
09:53
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
12.1K Views
09:56
Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
9.1K Views
10:02
piggyBac Transposon System Modification of Primary Human T Cells
17.2K Views
10:31
Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model
7.6K Views
08:13
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
21.4K Views
12:58
Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
9.3K Views
06:06
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
7.0K Views
09:54
Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
8.1K Views
12:49
Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
2.3K Views
Adult Pig Islet Isolation
2.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved