Results: PCR Validation of Porcine Specific Primers
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Conclusion
Transcription
Xenotransplantation is a feasible method to to treat However, effective monitoring of the immune rejection of xenotransplantation is a problem for physicians and the researchers. This protocol is a simple, convenient, low cost and less invasive me
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In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.