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Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

DOI :

10.3791/61599-v

11:40 min

January 21st, 2021

January 21st, 2021

4,169 Views

1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota

This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.

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Neural Stem Cells

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