Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
7.3K Views
•
13:29 min
•
January 16th, 2021
DOI :
January 16th, 2021
•Aigerim S. Kamzina*1,2,3, Michelle P. DiPalma*1,2,3, Joseph G. L. Hunter1,2,3, Andrew G. Diamos1,2,4, Boyd Armer2,3, Tsafrir S. Mor1,2,3, Hugh S. Mason1,2,3
1Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University, 2School of Life Sciences, Arizona State University, 3Molecular Biosciences/Biotechnology Undergraduate Program, Arizona State University, 4Department of Microbiology and Immunology, University of Michigan Medical School
Chapters in this video
0:00
Introduction
0:38
Cultivate Nicotiana benthamiana Plants
1:53
Preparation of Agrobacterium tumefaciens for Infiltration
3:51
Transient Needle-less Agroinfiltration
5:24
Grow and Observe the Infiltrated N.benthamiana
6:10
Protein Extraction
7:56
Protein G Column Chromatography
11:23
Representative Results
12:56
Conclusion
Related Videos
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
20.6K Views
08:56
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
17.2K Views
09:45
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
9.6K Views
08:07
Identification of Plasmodesmal Localization Sequences in Proteins In Planta
8.2K Views
08:21
Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
25.3K Views
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
49.0K Views
23:21
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
41.3K Views
11:10
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
20.8K Views
09:11
A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
23.4K Views
12:09
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
10.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved