Preparation of Agrobacterium tumefaciens for Infiltration
3:51
Transient Needle-less Agroinfiltration
5:24
Grow and Observe the Infiltrated N.benthamiana
6:10
Protein Extraction
7:56
Protein G Column Chromatography
11:23
Representative Results
12:56
Conclusion
Transcript
This is a demonstration of a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in tobacco related Nicotiana benthamiana plants. This protocol can be utilized for purification and visualization of plan
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We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana benthamiana. This protocol can be extended to purification and visualization of numerous proteins that utilize column chromatography. Moreover, the protocol is adaptable to the in-person and virtual college teaching laboratory, providing project-based exploration.