October 27th, 2020
•We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.
Related Videos
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures Video (Video) | JoVE
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells Video (Video) | JoVE
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish Video (Video) | JoVE
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol Video (Video) | JoVE
Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels Video (Video) | JoVE
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods Video (Video) | JoVE
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors Video (Video) | JoVE
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells Video (Video) | JoVE
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model Video (Video) | JoVE
Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved