Filling the Ion-Selective and Reference Microelectrode for ISME and SIET
3:25
Calibrating Electrodes for ISME and ISME Recordings
5:02
SIET Measurements
7:33
Isolated Hindgut Bioassay
8:26
Results: In Vitro Fluid Secretion Rate, Na+ Concentration and Transport Rate of Adult Female A. aegypti MTs
10:46
Conclusion
Transcript
This protocol describes techniques that can be utilized by small animal physiologists to elucidate the role of neuropeptides and other hormonal factors that regulate the digestive and or excretory system. These techniques allow for measurements of
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This protocol outlines methodologies behind the Ramsay assay, ion-selective microelectrodes, Scanning Ion-selective Electrode Technique (SIET) and in vitro contraction assays, applied to study the adult mosquito excretory system, comprised of the Malpighian tubules and hindgut, to collectively measure ion and fluid secretion rates, contractile activity, and transepithelial ion transport.