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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

6.3K Views

10:37 min

March 14th, 2021

DOI :

10.3791/61879-v

March 14th, 2021


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3D Cardiac Microtissue

Chapters in this video

0:04

Introduction

0:47

Casting of the Cardiac Microtissue Molds and Cell Seeding

3:32

Fixation and Permeabilization of the Cells and the Cardiac Microtissues for Immunostaining

5:45

Digestion of the Cardiac Microtissues and Cell Preparation for the Flow Cytometry

7:34

Results: Estimation of the Purity, Morphology, the Contraction Profile of the Cells in the Cardiac Microtissues

9:47

Conclusion

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