Separation of the Tongue Epithelium from the Mesenchyme/Underlying Connective Tissue
3:51
Cell Dissociation
5:06
Results: Effect of Cell Dissociation on Total Cell Number and Viability
6:29
Conclusion
Transcript
High yield and quality of cells from complex tissues are essential to commonly used experimental analysis, such as single cell RNA sequencing and primary stem cell cultures. This protocol generates healthy cells at high yield and quality from diff
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We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.