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Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

2.8K Views

13:13 min

March 19th, 2021

DOI :

10.3791/62308-v

March 19th, 2021


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Time lapse Imaging

Chapters in this video

0:04

Introduction

0:58

Adeno-Associated Virus (AAV) Preparation and Intravitreal Injection of Neonatal Mice

3:13

Retinal Dissections for Live-Imaging Experiment

5:04

Retinal Flat-Mount Preparation

6:35

Time-Lapse Confocal Imaging of Live Whole-Mount Retina Preparations

8:33

Image Deconvolution and Post-Processing in ImageJ

11:16

Results: Representative Results After Neonatal Intraocular Injection, Time-Lapse Imaging, and Deconvolution

12:13

Conclusion

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