Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
4.3K Views
•
06:00 min
•
September 20th, 2021
DOI :
September 20th, 2021
•Janna Leiz*1,2, Christian Hinze*1,2, Anastasiya Boltengagen3, Caroline Braeuning4, Christine Kocks3, Nikolaus Rajewsky3, Kai M. Schmidt-Ott1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Chapters in this video
0:04
Introduction
0:41
Tissue Preparation
1:41
Tissue Homogenization and Cell Lysis
3:11
Nuclei Purification
4:40
Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
5:30
Conclusion
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