JoVE Logo
Faculty Resource Center

Sign In

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

DOI :

10.3791/64165-v

9:28 min

August 9th, 2022

August 9th, 2022

1,491 Views

1Leonard Davis School of Gerontology, University of Southern California, 2Molecular and Computational Biology Department, USC Dornsife College of Letters, Arts and Sciences, 3Biochemistry and Molecular Medicine Department, USC Keck School of Medicine, 4Epigenetics and Gene Regulation, USC Norris Comprehensive Cancer Center, 5USC Stem Cell Initiative

Here we present a protocol to isolate nuclei from the brains of the short-lived vertebrate model Nothobranchius furzeri for downstream applications such as single-nucleus RNA sequencing or single-nucleus assay for transposase-accessible chromatin with sequencing (ATAC-seq).

Tags

Nuclei Isolation

-- Views

Related Videos

article

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

article

Author Spotlight: A Unique Mouse Model of Asphyxia-Induced Cardiac Arrest

article

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

article

In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy

article

Author Spotlight: Innovative Techniques and Future Directions in Stroke Research

article

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

article

Using the Chick Embryo Brain as a Model for In Vivo and Ex Vivo Analyses of Human Glioblastoma Cell Behavior

article

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

article

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

article

Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved