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Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

5.6K Views

11:11 min

October 8th, 2021

DOI :

10.3791/63049-v

October 8th, 2021


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Stereotaxic Viral Injection

Chapters in this video

0:05

Introduction

1:09

Stereotaxic Injection of Virus in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain

2:28

Gradient Index (GRIN) Lens Implantation in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain

6:47

Affixing Miniscope Holder (Base) to the Mouse Skull

7:53

Miniscope Mounting and In Vivo Ca2+ Imaging

9:37

Results: In Vivo Calcium Imaging Using a Miniscope

10:32

Conclusion

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