Biosurfactants are the empathic surface active molecules produced by microorganisms that have the capacity to reduce the surface tension of the liquid and the interficial tension between two different phases. The empathic nature of these molecules
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We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.