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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis Video (Video) | JoVE

DOI :

10.3791/63834-v

April 1st, 2022

April 1st, 2022

1,669 Views

1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus

The protocol presents a method for isolating whole cell protein lysates from dissected mouse embryo facial processes or cultured mouse embryonic palatal mesenchyme cells and performing subsequent western blotting to assess phosphorylated protein levels.

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